1. České Budějovice
http://www.c-budejovice.cz/en
GPS 48.9748N, 14.4744E
A town monuments reserve; the 133 x 133-metre Přemysl Otakar II. Square, a Renaissance town hall (1555), rebuilt in the Baroque style, the Samson’s Fountain (1720–27, the largest in the Czech Republic), a Gothic-Renaissance Černá (Black) Tower (72 m), a three-nave St. Nicholas Cathedral. The first horse-drawn railway on the European continent from České Budějovice to Linz was opened in 1832.

2. Český Krumlov
http://www.ckrumlov.info/docs/en/kaktualita.xml
GPS 48.8128N, 14.3171E
A picturesque town in the foothills of Šumava Mountains, included in the UNESCO‘s World Heritage List. Originally a 13th century Gothic castle was rebuilt into a Renaissance chateau. The remains of the fortification are interesting and also the characteristic quarter Latrán dating from the 13th century, originally a craft and trade centre. The originally Early Gothic Church of St. Vitus is valuable as well as the group of Gothic and Renaissance houses.

3. Tábor
http://www.taborcz.eu
GPS 49.4141N, 14.6587E
A main heavily fortified centre of the Czech Hussites; a part of town fortifications, the remains from the original Kotnov Castle, 
a late-Gothic town hall (Hussite Museum), the Church of the Transformation of Our Lord, 
a statue of J. Žižka (1884).

4. Jindřichův Hradec
http://infocentrum.jh.cz/en/
GPS 49.1442N, 15.0030E
A residence of the powerful family of Lords of Hradec, 
a town monuments reserve; 
the chateau complex, the Church of the Assumption of Our 
Lady (14th century), 
Minorite Monastery 
with the Church of St. John the Baptist, the town 
museum (in the collections, the mechanical nativity Scene 
constructed by Krýza, among others).

5. Třeboň
www.mesto-trebon.cz
GPS 49.0037N, 14.7713E
A town monuments reserve; a Renaissance chateau (1562), the Gothic Augustinian Monastery from the 14th century with the Church of St. Giles. Two large lakes, Svět and Opatovický, are on the edge; a neo-Gothic Schwarzenberg Tomb with a 19th century chapel lies between them.

6. Písek
http://www.pisek.eu/en/
GPS 49.3085N, 14.1464E
A town monuments zone; a stone bridge (the Czech oldest preserved), the remains of the early-Gothic Royal Castle (museum), the Church of the Birth of Our Lady (13th century).

7. Zvíkov
https://www.hrad-zvikov.eu/en
GPS 49.4387N, 14.1923E
A castle perched on a narrow buttress above the confluence of the Vltava and Otava rivers. It was founded as the royal castle in the first half of the 13th century, significantly enlarged in the Gothic and Renaissance styles.

8. Slavonice
http://www.i.slavonice-mesto.cz/en/
GPS 48.9976N, 15.3521E
A town monuments reserve; the collection of late-Gothic and Renaissance buildings with richly curved gables and with facades decorated by the sgraffito, two Gothic churches – of the Assumption of Our Lady and of St. John the Baptist. The pilgrimage Church of the Holy Spirit and Corpus Christi atop a hill above the town.

9. Prachatice
www.prachatice.eu
GPS 49.0127N, 13.9977E
A town monuments reserve; an original Gothic-Renaissance appearance, a town walls with cylindrical bastions and a double gate, the Church of St. James (14th century), but was rebuilt in 1410–1513.

10. Hluboká nad Vltavou
https://en.wikipedia.org/wiki/Hlubok%C3%A1_nad_Vltavou
GPS 49.0506N, 14.4421E
A magnificent chateau, originally the 13th-century Gothic castle, rebuilt in the Renaissance and Baroque style, it received its current English-Tudor (Windsor) neo-Gothic appearance during 1839–71. A gallery of the famous South Bohemian painter M. Aleš in the former riding-hall.

11. Červená Lhota
https://www.zamek-cervenalhota.cz/en
GPS 49.2466N, 14.8852E
A chateau on a rocky island in the middle of 
a lake. It was rebuilt from an original Gothic stronghold after 1530, the Renaissance and Rococo interiors.

12. Holašovice
www.holasovice.eu
GPS 48.9708N, 14.2731E
This charming village with a regular rectangular green and one of the best complexes of the Czech folk architecture has been listed with UNESCO. Farmhouses and granaries with richly curved and deco-rated gables share walls with gates.

13. Strakonice
http://www.strakonice.eu/en
GPS 49.2583N, 13.9030E
A Romanesque-Gothic castle (13th–14th century), renovated and enlarged in the Renaissance and Baroque styles, a former Butchers‘ Shop, a Church of St. Margaret from 1583.

14. Vimperk
http://www.vimperk.cz/en/tourism-in-vimperk/27/
GPS 49.0526N, 13.7737E
The early-Gothic castle, rebuilt in the Renaissance and Baroque styles (the Museum of the Šumava National Park and Protected Landscape Area), a remnants of fortifications with bastions and Černá (Black) Gate, log-built houses (17th–18th century), Church of the Visitation of Our Lady (13th–16th centuries) with the late-Gothic belfry called Černá (Black) Tower.

15. Bohemian Forest (Šumava)
http://www.npsumava.cz/en/
The south-east half of this mountain area, including part of the Šumava National Park and Protected Landscape Area. It is an area of extensive high bogs, large forest complexes of often wild almost virgin forest-like nature, mountain meadows with rich flora, deep river and stream valleys, massive rocks and rock seas. The highest peak of the Czech part of the Šumava – Plechý (1378 m), below it Plešné Lake.

16. Lipno
https://www.lipno.info/en/
GPS 48.7063N, 14.0853E
The 48-km long and 4780-ha water dam is the largest Czech artificial lake, also called ‘the Šumava Sea’, It was constructed in 1950–59. A centre of summer recreation.

17. Lenora
http://www.sumavanet.cz/lenora/fr.asp?tab=snet&id=5266&navrat=no
GPS 48.9227N, 13.7988E
A typical local village, log-built houses, a chateau (hotel),a covered wooden bridge, a bread baking oven from the fist half of the 19th century, a glasswork museum.

18. Volary
www.mestovolary.cz
GPS 48.9114N, 13.8919E
A collection of Alpine style buildings – log-built and partially wooden houses built by immigrants from Styria and Tyrol in the 16th century.

19. Vyšší Brod
http://www.klastervyssibrod.cz/[en]
GPS 48.6206N, 14.3054E
The Cistercian Monastery, was founded by the Rožmberks in 1259, rebuilt and enlarged in the 14th century and in the Baroque style between the 17th and 18th centuries, three-nave early-Gothic Church of the Assumption of Our Lady with the Rožmberks Tomb. In the second abbey house (built in 1641), Postal Museum.

20. Orlík
http://www.zamekorlik.cz/en/
GPS 49.5131N, 14.1704E
This originally Gothic castle once perched on a steep rock above the Vltava River is now surrounded by the charming Orlická Dam (1962). It was rebuilt several times during the centuries, its current romantic neo-Gothic appearance (19th century). A large park with a conservatory adjoins the chateau.

21. Blatná
https://en.wikipedia.org/wiki/Blatn%C3%A1
GPS 49.4227N, 13.8795E
A romantic water-castle, rebuilt in the 16th century in the Renaissance style and later received neo-Gothic face-lift, a late-Gothic Church of the Assumption of Our Lady (15th–16th century). Romantische Wasserburg, im 16. Jhd. im Renaissancestil umgebaut und neugotisch umgestaltet, spätgotische Kirche Mariä Himmelfahrt aus dem 15.-16. Jhd.

22. Choustník
www.obec-choustnik.cz
GPS 49.3329N, 14.8378E
A large well-maintained ruin of a castle from the second half of the 13th century with two separated cores – double-castles. It was rebuilt and newly fortified in the 15th and 16th century. A quadrant tower offers fine views.

23. Bechyně
http://www.mestobechyne.cz/en/english/the-town-of-bechyne/
GPS 49.2945N, 14.4677E
A spa town with a Renaissance chateau. In the town there are Gothic and Baroque churches, a town museum, the first Czech Fire Brigade Museum. The complex of spa buildings is from the 19th and 20th centuries; the Duha Bridge (1926–28) has one large 90-meter.

24. Milevsko
http://www.czechtourism.com/c/milevsko-monastery/
GPS 49.4521N, 14.3615E
A town with the Romanesque Premonstratensian Monastery from around 1187, partially rebuilt in the Baroque style (1761–67), the Minster of the Visitation of Our Lady, the Monastery now houses a museum; a Romanesque cemetery church from before 1150, rebuilt in the Gothic style.

25. Soběslav-Veselí Moorland (Soběslavsko-Veselská blata)

https://www.visittabor.eu/surroundings/zaluzi-forge-puppet-museum?backlink=du7lt
GPS 49.2487N, 14.6574E
Extensive fens surrounded by villages with charming folk architecture – farmhouses with granaries and gates from the 19th century. Most buildings are protected monuments zones or reserves. Among the most beautiful are Borkovice, Mažice, Zálší, Klečaty, Komárov, Svinky, Vlastiboř, Záluží, Nedvědice, Debrník and Bechyňská Smoleč.

26. Devil‘s wall (Čertova stěna)
https://rock-certova-stena.czech-mountains.eu
GPS 48.6308N, 14.2780E
A national nature reserve of fissured quadrant granite rocks above a deep valley of the Vltava River. Spectacular view from the top.

27. Soběslav
www.musobeslav.cz
GPS 49.2593N, 14.7210E
A town monuments zone; a late-Gothic cylindrical tower is preserved from the castle from 
the end of the 13th century; the Gothic Church of Sts. Peter and Paul (13th–15th century), the former hospital Church of St. Vitus.

28. Dačice
http://www.dacice.cz/en/
GPS 49.0796N, 15.4365E
The first world cube of sugar was being produced from 1843 in this town. A town monuments zone; the Renaissance Old Chateau, the Baroque Church of St. Lawrence with frescoes by J. Winterhalder, a Renaissance tower (51 m), the Empire New Chateau – open to the public.

29. Týn nad Vltavou
http://www.tnv.cz/en/
GPS 49.2237N, 14.4210E
A Renaissance salt-house, remains of castle in 1645, a Baroque chateau from 1699 (museum), a Renaissance town hall, the Gothic Church of St. James rebuilt in the Renaissance style.

30. Kratochvíle
https://www.zamek-kratochvile.cz/en
GPS 49.0590N, 14.1683E
The Renaissance Italian villa-style hunting chateau surrounded by a moat was ordered by Vilém of Rožmberk the end of the 16th century (an exposition – the history of Czech animated film).

31. Kleť
http://www.klet.org
GPS 48.8655N, 14.2832E
The highest peak (1038 m) of Blanský les (Blanský Forest). The oldest Czech stone lookout tower from 1825, an observatory (1957–59) open to the public.

32. Zlatá Koruna

http://www.ckrumlov.info/docs/de/region_histor_klaszk.xml
GPS 48.8552N, 14.3710E
The Cistercian Monastery founded in 1263; the complex of Gothic buildings with Baroque facelifts, the most valuable are the 14th– century Church of the Assumption of Our Lady and the 13th-century chapter.

33. Schwarzenberg navigation canal (Schwarzenberský plavební kanál)
www.schw-kan.com
GPS 48.6544N, 14.0350E
This 45-kilometre long technical monument (1789–1822) connects the Vltava and Danube rivers and is used for transport of wood from hardly accessible mountains areas.

34. Rožmberk
https://en.wikipedia.org/wiki/Ro%C5%BEmberk_Pond
GPS 49.0480N, 14.7616E
The 720-ha lake is the largest in Bohemia and Central Europe (1584–90).

35. Nové Hrady
http://www.novehrady.cz/
GPS 48.7902N, 14.7779E
A town with a Gothic castle (13th century), rebuilt in the Renaissance style in the 16th century and received a Baroque face-lift, the Gothic Church of Sts. Peter & Paul, a Renaissance town hall, unaccessible the empire chateau (1801–10).

36. Římov
https://en.wikipedia.org/wiki/Ř%C3%ADmov_(České_Budějovice_District)
GPS 48.8567N, 14.4885E
The pilgrimage place built by the Jesuits (1648–1705), a Loreto chapel (1648–53), the Church of the Holy Spirit was finished in 1672, the Jesuit residence (later a chateau) in 1685. A Stations of the Cross, called Římov Passion.

37. Rožmberk nad Vltavou
https://www.hrad-rozmberk.cz/en
GPS 48.6552N, 14.3668E
The original Upper castle was founded as the seat of the Rožmberk family before 1250; the 14th century Lower castle below was rebuilt in the 16th century and once more, in the Romanticism Gothic style (1840–57).

38. Helfenburk
https://en.wikipedia.org/wiki/Helfenburk
GPS 49.1359N, 14.0067E
One of the largest Czech castles built by the Rožmberk family (1360–70).

39. Boubín virgin forest (Boubínský prales)
http://www.npsumava.cz/en/3045/9980/clanek/
GPS 48.9746N, 13.8138E
The 620-ha natural reserve. The oldest trees are 300-400 years old; some have unusual deformations, tumours and harp-like formations, spruces on widely straddled stilt roots.

40. Lomec
http://www.lomec.cz/en/
GPS 49.0955N, 14.1876E
The Baroque pilgrimage Church of the Name of Our Lady with the imitation by the pope altar in the St. Peter Cathedral in Vatican.